![]() Of these markers, CD86 proved to be the most reliable since it detected all sensitizers, including benzocaine, a classical false negative in local lymph node assay (LLNA) but not irritants. Then, we tested MUTZ- 3 cells against a wider panel of sensitizers and irritants on a broader spectrum of cell surface markers (HLA-DR, CD40, CD54, CD80, CD86, B7-H 1, B7-H2, B7-DC). ![]() Only MUTZ- 3 and THP- 1 cells show a strong and specific response to sensitizer, while other cell lines showed very variable responses. First, we compared undifferentiated MUTZ- 3 cells with several standard human cells such as THP- 1, KG- 1, HL-60, K-562, and U- 937 in their response to the strong sensitizer DNCB and the irritant SDS by monitoring the expression levels of HLA-DR, CD54, and CD86 by flow cytometry. In this study, we have evaluated the performances of MUTZ- 3, a cytokine-dependent human monocytic cell line, in its response to sensitizers. ![]() However, no in vitro methods exist which are based on the activation process of LC to predict the sensitization potential of chemicals. Langerhans cells (LC) are key mediators of contact allergenicity in the skin. International Nuclear Information System (INIS)Īzam, Philippe Peiffer, Jean-Luc Chamousset, Delphine Tissier, Marie-Helene Bonnet, Pierre-Antoine Vian, Laurence Fabre, Isabelle Ourlin, Jean-Claude The cytokine-dependent MUTZ- 3 cell line as an in vitro model for the screening of contact sensitizers
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